{"product_id":"elk5675","title":"Dog GMCSF(Colony Stimulating Factor 2, Granulocyte Macrophage) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e CSF2; GM-CSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 3.2 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 500 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 7.82-500 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Cytokine;Hematology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog GMCSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GMCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog GMCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog GMCSF in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P48749","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410022404376,"sku":"ELK5675","price":688.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_e3758a1f-a5ed-4003-92d6-5bd3fd4236f3.jpg?v=1751014630","url":"https:\/\/danabiosci.com\/products\/elk5675","provider":"Dana Bioscience","version":"1.0","type":"link"}