{"product_id":"elk5367","title":"Human MEP1a(Meprin A Alpha) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 17 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 4000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 62.5-4000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MEP1a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q16819","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50410003988760,"sku":"ELK5367","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_84e99e40-d097-4b5d-9b59-b324b53e852f.jpg?v=1751013986","url":"https:\/\/danabiosci.com\/products\/elk5367","provider":"Dana Bioscience","version":"1.0","type":"link"}