{"product_id":"elk4193","title":"Human ANLN(Anillin) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Scra; Scraps; Actin-Binding Protein Anillin\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 12 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 2000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 31.25-2000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Tissue homogenates, cell lysates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ANLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANLN in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q9NQW6","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409873047832,"sku":"ELK4193","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_99c57be4-fd41-4955-9872-e117469669a0.jpg?v=1751010696","url":"https:\/\/danabiosci.com\/products\/elk4193","provider":"Dana Bioscience","version":"1.0","type":"link"}