{"product_id":"elk4069","title":"Human APLN(Apelin) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e XNPEP2; APEL; APJ endogenous ligand\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 8.4 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 1600 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 25-1600 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Endocrinology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APLN in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q9ULZ1","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409860825368,"sku":"ELK4069","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_1c8f061d-54a9-4e2e-94f9-4f6259d1c38a.jpg?v=1751010319","url":"https:\/\/danabiosci.com\/products\/elk4069","provider":"Dana Bioscience","version":"1.0","type":"link"}