{"product_id":"elk3677","title":"Human CER(Cerberus) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e CER1; DAND4; Cerberus 1; DAN domain family member 4; Cysteine Knot Superfamily,Homolog(Xenopus Laevis); Cerberus-related protein\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 13 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 2000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 31.25-2000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Cytokine;Signal transduction;Developmental science;Bone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CER in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e O95813","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409827205400,"sku":"ELK3677","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_f1c6c2a5-e2ff-4c8e-9c52-05b959e7a6c1.jpg?v=1751009157","url":"https:\/\/danabiosci.com\/products\/elk3677","provider":"Dana Bioscience","version":"1.0","type":"link"}