{"product_id":"elk3318","title":"Human SPON1(Spondin 1) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e VSGP; F-spondin; F-spondin,Extracellular Matrix Protein; Vascular smooth muscle cell growth-promoting factor\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.236 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 40 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.63-40 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPON1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPON1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q9HCB6","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409805644056,"sku":"ELK3318","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_4b22d892-e916-43f6-a2be-58dbb1aca303.jpg?v=1751008121","url":"https:\/\/danabiosci.com\/products\/elk3318","provider":"Dana Bioscience","version":"1.0","type":"link"}