{"product_id":"elk3110","title":"Human COL11a1(Collagen Type XI Alpha 1) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e COL11-A1; CO11A1; COLL6; STL2; Collagen Alpha-1(XI)chain\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 15 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 2500 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 39.07-2500 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL11a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL11a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL11a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL11a1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P12107","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409791521048,"sku":"ELK3110","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_476f9e45-faea-416f-9d3c-9d5aa5921bae.jpg?v=1751007515","url":"https:\/\/danabiosci.com\/products\/elk3110","provider":"Dana Bioscience","version":"1.0","type":"link"}