{"product_id":"elk2635","title":"Human BMP7(Bone Morphogenetic Protein 7) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e OP1; Osteogenic Protein-1; Eptotermin alfa\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 27.4 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 4000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 62.5-4000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;Developmental science;Bone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP7 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P18075","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409762128152,"sku":"ELK2635","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_e277e27d-f7cb-4cfc-ab63-c19fd55841ef.jpg?v=1751006260","url":"https:\/\/danabiosci.com\/products\/elk2635","provider":"Dana Bioscience","version":"1.0","type":"link"}