{"product_id":"elk2631","title":"Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase\/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.109 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 20 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.32-20 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e tissue homogenates, cell lysates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Enzyme \u0026amp; Kinase;Neuro science;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q6UWV6","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409761964312,"sku":"ELK2631","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_0568e745-b800-4b1c-a54d-7b0f78790955.jpg?v=1751006248","url":"https:\/\/danabiosci.com\/products\/elk2631","provider":"Dana Bioscience","version":"1.0","type":"link"}