{"product_id":"elk2436","title":"Rat ET-1(Endothelin1) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 2.63 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 500 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 7.82-500 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Endocrinology;Cardiovascular biology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P22387","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409751773464,"sku":"ELK2436","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_4b5c59b8-e3f3-43ce-bf3d-b0cbe8ba31d1.jpg?v=1751005807","url":"https:\/\/danabiosci.com\/products\/elk2436","provider":"Dana Bioscience","version":"1.0","type":"link"}