{"product_id":"elk10835","title":"Rat PCSK6(Proprotein Convertase Subtilisin\/Kexin Type 6) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e PACE4; SPC4; Paired Basic Amino Acid Cleaving System 4; Subtilisin-Like Proprotein Convertase 4; Subtilisin\/Kexin-Like Protease PACE4\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 1.23 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 200 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 3.13-200 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e tissue homogenates, cell lysates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Enzyme \u0026amp; Kinase;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCSK6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCSK6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PCSK6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PCSK6 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q63415","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409577218328,"sku":"ELK10835","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_cd052df8-42f7-462f-8d4c-4e9adf4fae6b.jpg?v=1751001420","url":"https:\/\/danabiosci.com\/products\/elk10835","provider":"Dana Bioscience","version":"1.0","type":"link"}