{"product_id":"elk10370","title":"Human MHCE(Major Histocompatibility Complex Class I E) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.057 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 10 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.16-10 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Developmental science;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCE in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P13747","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409510863128,"sku":"ELK10370","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_b32e8411-9278-4681-89fc-ec5ec464d382.jpg?v=1750999930","url":"https:\/\/danabiosci.com\/products\/elk10370","provider":"Dana Bioscience","version":"1.0","type":"link"}