{"product_id":"elk10312","title":"Rat TSP-1(Thrombospondin-1) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.46 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 100 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 1.56-100 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Plasma\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Infection immunity;Hematology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e Q71SA3","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409502998808,"sku":"ELK10312","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_8d7400cb-11ff-4466-81fb-b46aab6c63ef.jpg?v=1750999746","url":"https:\/\/danabiosci.com\/products\/elk10312","provider":"Dana Bioscience","version":"1.0","type":"link"}