{"product_id":"elk0960","title":"Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Bsk; BSK; CG5680; D-JNK; D-junk; DBSK\/JNK; dJnk; dJNK; DJNK; DJNK\/bsk; Dmel\\CG5680; DmJNK; dNJNK; jnk; Jnk; JNK; jnk (bsk); JNK\/SAPK; Junk; p-JNK; P-JNK; pJNK; SAPKa\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.148 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 20 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.32-20 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e tissue homogenates, cell lysates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Enzyme \u0026amp; Kinase;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409455616280,"sku":"ELK0960","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_0e85cf0e-a0ec-4ad7-92ec-cc0dbe8a7a8b.jpg?v=1750998737","url":"https:\/\/danabiosci.com\/products\/elk0960","provider":"Dana Bioscience","version":"1.0","type":"link"}