{"product_id":"elk0812","title":"Rat Glu(Glutamic Acid) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Glutamate\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 50.1 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 10000 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 156.25-10000 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 2.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Enzyme \u0026amp; Kinase;Endocrinology;Neuro science;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Glu protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Glu. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Glu in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409420259608,"sku":"ELK0812","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_af5e4a2b-97f2-4b80-8041-fc8685530518.jpg?v=1750998309","url":"https:\/\/danabiosci.com\/products\/elk0812","provider":"Dana Bioscience","version":"1.0","type":"link"}