{"product_id":"elk0778","title":"Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e 2,3DPG\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.14 µmol\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 20 µmol\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.32-20 µmol\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates, cell lysates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409418555672,"sku":"ELK0778","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_783b2f94-5046-4119-93cb-902cd6ec54c8.jpg?v=1750998219","url":"https:\/\/danabiosci.com\/products\/elk0778","provider":"Dana Bioscience","version":"1.0","type":"link"}