{"product_id":"elk0752","title":"Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Anti-Tissue Transglutaminase IgA\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 1.45 IU\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 300 IU\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 4.69-300 IU\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma, tissue homogenates and other biological fluids.\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Enzyme \u0026amp; Kinase;Endocrinology;Neuro science;Autoimmunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409416327448,"sku":"ELK0752","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_8ebb6806-cfb8-46b5-bf27-b20181272968.jpg?v=1750998151","url":"https:\/\/danabiosci.com\/products\/elk0752","provider":"Dana Bioscience","version":"1.0","type":"link"}