{"product_id":"elk059es","title":"EasyStep Human IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.98 pg\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 5000 pg\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 78.13-5000 pg\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 1.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;Tumor immunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGFBP3, and the Human IGFBP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IGFBP3. After TMB substrate solution is added, only those wells that contain Human IGFBP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409335292184,"sku":"ELK059ES","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_7ec3ed7e-587e-45db-9f59-7fcdfb2938d7.jpg?v=1750996035","url":"https:\/\/danabiosci.com\/products\/elk059es","provider":"Dana Bioscience","version":"1.0","type":"link"}