{"product_id":"elk051es","title":"EasyStep Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e 25 Hydroxy Vitamin D;\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 7.7 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 5000 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 78.13-5000 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 1.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409334636824,"sku":"ELK051ES","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_446b2743-7651-452e-8ebd-5da8e51be447.jpg?v=1750996013","url":"https:\/\/danabiosci.com\/products\/elk051es","provider":"Dana Bioscience","version":"1.0","type":"link"}