{"product_id":"elk048es","title":"EasyStep Human fT3(Free Triiodothyronine) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Competitive Inhibition\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.31 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 20 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.32-20 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e Serum, plasma\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 1.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Endocrinology;Autoimmunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT3 antigen, and the Human fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard\/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409333621016,"sku":"ELK048ES","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_1c074f93-366e-49c3-9eae-4ade39dcc97f.jpg?v=1750996001","url":"https:\/\/danabiosci.com\/products\/elk048es","provider":"Dana Bioscience","version":"1.0","type":"link"}