{"product_id":"elk0453","title":"Sheep C3a(Complement Component 3a) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e Complement Component 3a\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 3.73 ng\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 600 ng\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 9.38-600 ng\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Infection immunity;Immune molecule;Autoimmunity;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep C3a in the samples is then determined by comparing the OD of the samples to the standard curve.","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":50409384444184,"sku":"ELK0453","price":558.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/ELK_56c552bc-8582-4cf8-8ec5-1902037ab297.jpg?v=1750997340","url":"https:\/\/danabiosci.com\/products\/elk0453","provider":"Dana Bioscience","version":"1.0","type":"link"}