{"product_id":"elisa-kit-for-wingless-type-mmtv-integration-site-family-member-5a-wnt5a-abcc24723","title":"ELISA Kit for Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e0.156-10ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.059ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eTissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e \u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eWingless Type MMTV Integration Site Family, Member 5A\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eSignal transduction;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25488180\"\u003eSecreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26547443\"\u003eWnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25488180%20\"\u003eSecreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26163991\"\u003eWnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29674475\"\u003eEffect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 0.059ng\/mL \/ Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)","offer_id":42481721442538,"sku":"ABCC24723","price":792.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_95fce869-8b85-4c35-9767-0c5764e6d479.png?v=1644865571","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-wingless-type-mmtv-integration-site-family-member-5a-wnt5a-abcc24723","provider":"Dana Bioscience","version":"1.0","type":"link"}