{"product_id":"elisa-kit-for-vitamin-b2-vb2-abcc23585","title":"ELISA Kit for Vitamin B2 (VB2)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e7.41-600ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 2.61ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin B2 (VB2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin B2 (VB2) and unlabeled Vitamin B2 (VB2) (Standards or samples) with the pre-coated antibody specific to Vitamin B2 (VB2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B2 (VB2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin B2 (VB2) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eRiboflavin; Riboflavine\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eVitamin B2\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29325932\"\u003eRiboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial …\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 2.61ng\/mL \/ Pan-species (General)","offer_id":42481583423722,"sku":"ABCC23585","price":880.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_85891ac5-5b9c-415c-a53e-9374a9c68e62.png?v=1644863727","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-vitamin-b2-vb2-abcc23585","provider":"Dana Bioscience","version":"1.0","type":"link"}