{"product_id":"elisa-kit-for-urocortin-2-ucn2-abcc24590","title":"ELISA Kit for Urocortin 2 (UCN2)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e0.78-50ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eRattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.31ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Urocortin 2 (UCN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Urocortin 2 (UCN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Urocortin 2 (UCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Urocortin 2 (UCN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eURP; SRP; UCN-II; UCNII; Stresscopin-Related Peptide; Urocortin-related peptide\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eUrocortin 2\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eEndocrinology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29584808\"\u003eUrocortin-2 improves right ventricular function and attenuates pulmonary arterial hypertension\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29604138\"\u003eGlucocorticoid withdrawal affects stress‐induced changes in urocortin 2 gene expression in the rat adrenal medulla and brain\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 0.31ng\/mL \/ Rattus norvegicus (Rat)","offer_id":42481698275562,"sku":"ABCC24590","price":760.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_5c1d6215-27cc-4120-85f2-bf3bc578a830.png?v=1644865361","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-urocortin-2-ucn2-abcc24590","provider":"Dana Bioscience","version":"1.0","type":"link"}