{"product_id":"elisa-kit-for-tumor-necrosis-factor-alpha-tnfa-abcc24345","title":"ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e15.6-1,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eMus musculus (Mouse), Rattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 6.2pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eDIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eTumor Necrosis Factor Alpha\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eCytokine;Tumor immunity;Infection immunity;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27283193\"\u003eAntiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27235741\"\u003eBexarotene protects against traumatic brain injury in mice partially through apolipoprotein E\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27473983\"\u003eA chimeric protein composed of the binding domains ofClostridium perfringens phospholipase C and Trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27026909\"\u003eEthyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1\/RAGE\/NF-ΚB pathway.\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26190035\"\u003eAmelioration of free fatty acid-induced fatty liver by quercetin-3-O-β-D-glucuronide through modulation ofperoxisome proliferator-activated receptor-alpha\/sterol reCavia (Guinea pig )latory element-binding protein-1c signaling.\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4834117\/\"\u003eNeuroprotective effects of atomoxetine against traumatic spinal cord injury in rats\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26935117\"\u003eCurcumin Attenuates Inflammation, Oxidative Stress, and Ultrastructural Damage Induced by Spinal Cord Ischemia–Reperfusion Injury in Rats\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26898127\"\u003eCarcinoembryonic antigen related cellular adhesion molecule 1 alleviates dextran sulfate sodium-induced ulcerative colitis in mice\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26706290\"\u003eCardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27110299\"\u003eThe bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27347929\"\u003eDexmedetomidine Protects Rat Liver against Ischemia-Reperfusion Injury Partly by the α2A-Adrenoceptor Subtype and the Mechanism Is Associated with the TLR4\/NF-κB Pathway\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29292463\"\u003eSea cucumber saponins liposomes ameliorate obesity-induced inflammation and insulin resistance in high-fat diet-fed mice\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29867706\"\u003eUrsolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1\/Toll-Like Receptor 4\/NFκB …\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29595858\"\u003eCalycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin\/AMPK\/eNOS pathway in obese mice\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29800788\"\u003eThe effect of natural antioxidants in cyclophosphamide-induced hepatotoxicity: Role of Nrf2\/HO-1 pathway\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29784871\"\u003eEffect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29984607\"\u003eShock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse model\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30005909\"\u003eAnti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ijcem.com\/files\/ijcem0067841.pdf\"\u003eImprovement effect of doxofylline on the mechanism of glucocorticoid resistance in chronic obstructive pulmonary disease rat model\u003c\/a\u003e;\u003ca href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/tox.22634\"\u003ePotential immunomodulatory and antioxidant effects of walnut  vegetable oil against lead‐mediated hepatic damage and their interaction with lipase …\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0024320518305435\"\u003eSitagliptin attenuates intestinal ischemia\/reperfusion injury via cAMP\/PKA, PI3K\/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ijcem.com\/files\/ijcem0067329.pdf\"\u003eThe relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6129538\/\"\u003eHuman mesenchymal stem cells attenuate hyperoxia-induced lung injury through inhibition of the renin-angiotensin system in newborn rats\u003c\/a\u003e;\u003ca href=\"https:\/\/www.hindawi.com\/journals\/omcl\/2018\/3509091\/abs\/\"\u003eVildagliptin Attenuates Hepatic Ischemia\/Reperfusion Injury via the TLR4\/NF-κB Signaling Pathway\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S1567576918306520\"\u003eNaringin protects against perfluorooctane sulfonate-induced liver injury by modulating NRF2 and NF-κB in mice\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0085253818308408\"\u003eWnt\/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome\u003c\/a\u003e;\u003ca href=\"https:\/\/www.mdpi.com\/2072-6643\/11\/8\/1728\"\u003eCardioprotective Effect of a Virgin Olive Oil Enriched with Bioactive Compounds in Spontaneously Hypertensive Rats\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 6.2pg\/mL \/ Mus musculus (Mouse), Rattus norvegicus (Rat)","offer_id":42481657413866,"sku":"ABCC24345","price":480.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_7ecef3c8-569d-40e5-938b-91c2a518dfc8.png?v=1644864988","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-tumor-necrosis-factor-alpha-tnfa-abcc24345","provider":"Dana Bioscience","version":"1.0","type":"link"}