{"product_id":"elisa-kit-for-sphingosine-1-phosphate-s1p-abcc23554","title":"ELISA Kit for Sphingosine-1-Phosphate (S1P)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e12.35-1,000ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 4.62ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Sphingosine-1-Phosphate (S1P) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Sphingosine-1-Phosphate (S1P) and unlabeled Sphingosine-1-Phosphate (S1P) (Standards or samples) with the pre-coated antibody specific to Sphingosine-1-Phosphate (S1P). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Sphingosine-1-Phosphate (S1P) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Sphingosine-1-Phosphate (S1P) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e \u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eSphingosine-1-Phosphate\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29940125\"\u003eQuercetin ameliorates pulmonary fibrosis by inhibiting SphK1\/S1P signaling\u003c\/a\u003e;\u003ca href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1111\/1440-1681.13124\"\u003eSPNS 2 promotes the malignancy of colorectal cancer cells via regulating Akt and ERK pathway\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 4.62ng\/mL \/ Pan-species (General)","offer_id":42481576870122,"sku":"ABCC23554","price":940.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_124247c9-8ccc-46d8-9e44-7a616bcdce8a.png?v=1644863673","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-sphingosine-1-phosphate-s1p-abcc23554","provider":"Dana Bioscience","version":"1.0","type":"link"}