{"product_id":"elisa-kit-for-secretory-immunoglobulin-a-siga-abcc25361","title":"ELISA Kit for Secretory Immunoglobulin A (sIgA)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e617.28-50000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eChicken (Gallus)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 248.5pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretory Immunoglobulin A (sIgA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretory Immunoglobulin A (sIgA) and unlabeled Secretory Immunoglobulin A (sIgA) (Standards or samples) with the pre-coated antibody specific to Secretory Immunoglobulin A (sIgA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretory Immunoglobulin A (sIgA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretory Immunoglobulin A (sIgA) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eS-IgA\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eSecretory Immunoglobulin A\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eInfection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24570422%20\"\u003eEffects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28938780\"\u003eEffects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 248.5pg\/mL \/ Chicken (Gallus)","offer_id":42481791009002,"sku":"ABCC25361","price":684.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_4ce07243-c3a5-4e6f-8266-e30ceea6c8e5.png?v=1644866495","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-secretory-immunoglobulin-a-siga-abcc25361","provider":"Dana Bioscience","version":"1.0","type":"link"}