{"product_id":"elisa-kit-for-s-adenosyl-methionine-sam-abcc25007","title":"ELISA Kit for S-Adenosyl Methionine (SAM)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e12.35-1,000ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 4.84ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to S-Adenosyl Methionine (SAM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled S-Adenosyl Methionine (SAM) and unlabeled S-Adenosyl Methionine (SAM) (Standards or samples) with the pre-coated antibody specific to S-Adenosyl Methionine (SAM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of S-Adenosyl Methionine (SAM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of S-Adenosyl Methionine (SAM) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eSAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eS-Adenosyl Methionine\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27929536\"\u003eHyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29232372vvvv\"\u003eAttenuated expression of MTR in both prenatally androgenized mice and women with the hyperandrogenic phenotype of PCOS\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29879990\"\u003eMetabolic engineering of Acremonium chrysogenum for improving cephalosporin C production independent of methionine stimulation\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 4.84ng\/mL \/ Pan-species (General)","offer_id":42481757552874,"sku":"ABCC25007","price":816.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_dcbb94c1-ba68-45fb-b7a4-07fd0e67fba0.png?v=1644865986","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-s-adenosyl-methionine-sam-abcc25007","provider":"Dana Bioscience","version":"1.0","type":"link"}