{"product_id":"elisa-kit-for-proliferating-cell-nuclear-antigen-pcna-abcc24896","title":"ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e0.156-10ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.055ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eTissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferating Cell Nuclear Antigen (PCNA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proliferating Cell Nuclear Antigen (PCNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proliferating Cell Nuclear Antigen (PCNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proliferating Cell Nuclear Antigen (PCNA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eCyclin\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eProliferating Cell Nuclear Antigen\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eTumor immunity;Developmental science;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0223523419305379\"\u003eSynthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity\u003c\/a\u003e;\u003ca href=\"https:\/\/www.mdpi.com\/2076-3921\/8\/8\/312\"\u003eThe Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 0.055ng\/mL \/ Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)","offer_id":42481741037802,"sku":"ABCC24896","price":648.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_2baf3528-48bf-40be-ab7d-54916297d658.png?v=1644865824","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-proliferating-cell-nuclear-antigen-pcna-abcc24896","provider":"Dana Bioscience","version":"1.0","type":"link"}