{"product_id":"elisa-kit-for-procollagen-i-n-terminal-propeptide-pinp-abcc24256","title":"ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e46.88-3,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eMus musculus (Mouse)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 17.71pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I N-Terminal Propeptide (PINP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen I N-Terminal Propeptide (PINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I N-Terminal Propeptide (PINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eP1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eProcollagen I N-Terminal Propeptide\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eMetabolic pathway;Hepatology;Bone metabolism;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/ediss.sub.uni-hamburg.de\/volltexte\/2016\/7910\/pdf\/Dissertation.pdf\"\u003eBilaterale Kommunikation zwischen Osteoblasten und Osteoklasten\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28303909\"\u003eSprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29119115\"\u003eOptimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S8756328218304691\"\u003eRapamycin improves bone mass in high-turnover osteoporosis with iron accumulation through positive effects on osteogenesis and angiogenesis\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 17.71pg\/mL \/ Mus musculus (Mouse)","offer_id":42481644667114,"sku":"ABCC24256","price":698.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_5fa1136f-18f6-4b3d-8e70-8575d6fb1087.png?v=1644864836","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-procollagen-i-n-terminal-propeptide-pinp-abcc24256","provider":"Dana Bioscience","version":"1.0","type":"link"}