{"product_id":"elisa-kit-for-n-terminal-pro-brain-natriuretic-peptide-nt-probnp-abcc24260","title":"ELISA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e78-5,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eMus musculus (Mouse)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 28pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eNT-Pro-BNP; ; N-BNP\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eN-Terminal Pro-Brain Natriuretic Peptide\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eEndocrinology;Cardiovascular biology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/academic.oup.com\/cardiovascres\/advance-article-abstract\/doi\/10.1093\/cvr\/cvz140\/5497483\"\u003eCardio-protective effect of the secretome of Sca-1+ and Sca-1-cells in heart failure–not equal, but equally important? Paracrine cell effects on heart failure\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 28pg\/mL \/ Mus musculus (Mouse)","offer_id":42481644798186,"sku":"ABCC24260","price":680.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_4dacc372-9b39-4d9c-ab7b-c0387022ea87.png?v=1644864842","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-n-terminal-pro-brain-natriuretic-peptide-nt-probnp-abcc24260","provider":"Dana Bioscience","version":"1.0","type":"link"}