{"product_id":"elisa-kit-for-insulin-ins-abcc25275","title":"ELISA Kit for Insulin (INS)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e123.5-10,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eBos taurus; Bovine (Cattle)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 47.1pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eInsulin\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eMetabolic pathway;Endocrinology;Hormone metabolism;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/ainfo.cnptia.embrapa.br\/digital\/bitstream\/item\/96833\/1\/Cardoso-metabolitos.pdf\"\u003eCORRELA??ES ENTRE METABóLITOS E HORM?NIOS NO PERíODO \nDE TRANSI??O DE VACAS DE CORTE DE DIFERENTES GENóTIPOS \nCRIADAS NO SUL DO BRASIL \u003c\/a\u003e;\u003ca href=\"http:\/\/www.alice.cnptia.embrapa.br\/bitstream\/doc\/979246\/1\/Cardosocctrcaoinsulina.pdf\"\u003eCONCENTRA??ES SéRICAS DE INSULINA NO PóS-PARTO RECENTE \nDE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO \nSUL DO BRASIL\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29477524\"\u003eEffects of dietary leucine and phenylalanine on pancreas development, enzyme activity, and relative gene expression in milk-fed Holstein dairy calves\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 47.1pg\/mL \/ Bos taurus; Bovine (Cattle)","offer_id":42481781178602,"sku":"ABCC25275","price":756.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_c4ec6983-2e69-4a02-bc9b-b57b776572d9.png?v=1644866372","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-insulin-ins-abcc25275","provider":"Dana Bioscience","version":"1.0","type":"link"}