{"product_id":"elisa-kit-for-insulin-ins-abcc25259","title":"ELISA Kit for Insulin (INS)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e61.7-5,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 25.3pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eserum, plasma, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin (INS) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eInsulin\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eMetabolic pathway;Endocrinology;Hormone metabolism;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3770514\/\"\u003eNicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway\u003c\/a\u003e;\u003ca href=\"http:\/\/www.usa-journals.com\/wp-content\/uploads\/2013\/09\/Zineldeen_Vol110.pdf\"\u003ePolo Like Kinase 1 (Plk1) Expression in Visceral Adipose Tissue of Morbidly Obese Non Diabetic Women\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24568505\"\u003eEffect of NUCKS-1 Overexpression on cytokine profiling in obese women with breast cancer\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24814037\"\u003eAntioxidative effects of Korean red ginseng in postmenopausal women: A double-blind randomized controlled trial\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ajol.info\/index.php\/ijbcs\/article\/view\/127127\"\u003eTaraxacum officinale\u003c\/a\u003e;\u003ca href=\"http:\/\/www.freepatentsonline.com\/y2015\/0301040.html\"\u003eIDENTIFICATION OF MODULATORS OF BINDING PROPERTIES OF ANTIBODIES REACTIVE WITH A MEMBER OF THE INSULIN RECEPTOR FAMILY\u003c\/a\u003e;\u003ca href=\"https:\/\/www.researchgate.net\/profile\/Muhammad_Saiedullah\/publication\/305790299_Low_Circulating_Vaspin_Level_is_not_associated_with_Insulinemic_Status_in_Impaired_Glucose_Tolerant_Subjects\/links\/57a1f09408aeb1604833d46e.pdf\"\u003eLow Circulating Vaspin Level is not associated with Insulinemic Status in Impaired Glucose Tolerant Subjects\u003c\/a\u003e;\u003ca href=\"http:\/\/www.jofem.org\/index.php\/jofem\/article\/view\/405\"\u003eCirculating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis\u003c\/a\u003e;\u003ca href=\"http:\/\/downloads.hindawi.com\/journals\/jdr\/aip\/2376016.pdf\"\u003eHigh plasma glucagon levels correlate with waist-to-hip ratio, supra-iliac skinfold thickness, deep subcutaneous abdominal and intra-peritoneal adipose tissue …\u003c\/a\u003e;\u003ca href=\"http:\/\/dx.doi.org\/10.1136\/bmjdrc-2017-000393\"\u003eHigh circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study\u003c\/a\u003e;\u003ca href=\"https:\/\/doi.org\/10.1155\/2017\/2376016\"\u003eHigh Plasma Glucagon Levels Correlate with Waist-to-Hip Ratio, Suprailiac Skinfold Thickness, and Deep Subcutaneous Abdominal and Intraperitoneal Adipose Tissue Depots in Nonobese Asian Indian Males with Type 2 Diabetes in North India\u003c\/a\u003e;\u003ca href=\"http:\/\/www.jcmrp.eg.net\/text.asp?2017\/2\/2\/125\/217737\"\u003eRelationship between serum leptin concentration and insulin resistance syndrome in patients with type 2 diabetes mellitus\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29137982\"\u003eAdipose tissue complement factor B promotes adipocyte maturation.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28584599\"\u003eThe Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29461972\"\u003eDiet enriched with fresh coconut decreases blood glucose levels and body weight in normal adults\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29805533\"\u003eLiraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29719598\"\u003eHyperandrogenism and insulin resistance contribute to hepatic steatosis and inflammation in female rat liver\u003c\/a\u003e;\u003ca href=\"http:\/\/search.ebscohost.com\/login.aspx?direct=true\u0026amp;profile=ehost\u0026amp;scope=site\u0026amp;authtype=crawler\u0026amp;jrnl=00433144\u0026amp;AN=133147220\u0026amp;h=X7LQ6%2B%2Be9ypxbWxzOmVPAQpCgp9oahzzYOTq20AfZFRjYJsdvpXasaiIbaB2NS05eS6Fyvjbj5jTO9olJltpoA%3D%3D\u0026amp;crl=c\"\u003ePlasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2214623719300444\"\u003eDobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism\u003c\/a\u003e;\u003ca href=\"https:\/\/www.tandfonline.com\/doi\/abs\/10.1080\/13813455.2019.1635622\"\u003eAssociations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training\u003c\/a\u003e;\u003ca href=\"https:\/\/www.mdpi.com\/2072-6643\/11\/9\/1981\"\u003eHigh-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 25.3pg\/mL \/ Homo sapiens (Human)","offer_id":42481779998954,"sku":"ABCC25259","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_c3a7dae6-3911-4a46-ac53-c933fac103bd.png?v=1644866355","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-insulin-ins-abcc25259","provider":"Dana Bioscience","version":"1.0","type":"link"}