{"product_id":"elisa-kit-for-glypican-4-gpc4-abcc24867","title":"ELISA Kit for Glypican 4 (GPC4)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e15.6-1,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eRattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 6.1pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates and other biological fluids.\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glypican 4 (GPC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glypican 4 (GPC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glypican 4 (GPC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glypican 4 (GPC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eK-Glypican; Glypican Proteoglycan 4; Secreted glypican-4\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eGlypican 4\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eTumor immunity;Developmental science;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/portal.research.lu.se\/portal\/files\/43061981\/250690_2_G5_Svajunas_S.pdf\"\u003eAspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions.\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 6.1pg\/mL \/ Rattus norvegicus (Rat)","offer_id":42481738252522,"sku":"ABCC24867","price":684.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_0b6d850d-0e19-4621-8c0c-ecf7c67106db.png?v=1644865777","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-glypican-4-gpc4-abcc24867","provider":"Dana Bioscience","version":"1.0","type":"link"}