{"product_id":"elisa-kit-for-cytokeratin-15-ck15-abcc24823","title":"ELISA Kit for Cytokeratin 15 (CK15)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e31.2-2,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 12.7pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eserum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 15 (CK15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 15 (CK15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 15 (CK15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytokeratin 15 (CK15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eKRT15; K15, CK15, K1CO; KRTB; Keratin 15; Keratin-15,Basic; Keratin-15,Beta; Keratin, type I cytoskeletal 15\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eCytokeratin 15\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eTumor immunity;Infection immunity;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29749501\"\u003ePlacental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 12.7pg\/mL \/ Homo sapiens (Human)","offer_id":42481732223210,"sku":"ABCC24823","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_c248fc91-75dc-48c5-bfd1-5bcce9251ebb.png?v=1644865720","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-cytokeratin-15-ck15-abcc24823","provider":"Dana Bioscience","version":"1.0","type":"link"}