{"product_id":"elisa-kit-for-cytochrome-c-oxidase-subunit-ii-cox2-abcc24738","title":"ELISA Kit for Cytochrome C Oxidase Subunit II (COX2)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e0.156-10ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.054ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003etissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit II (COX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit II (COX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit II (COX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit II (COX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eMTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eCytochrome C Oxidase Subunit II\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eEnzyme \u0026amp; Kinase;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28174048\"\u003eThe effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29313174\"\u003ePolar extract of  protects cartilage homeostasis: possible mechanism of action\u003c\/a\u003e;\u003ca href=\"https:\/\/doi.org\/10.2174\/1573401313666170427123153\"\u003eBarrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro\u003c\/a\u003e;\u003ca href=\"https:\/\/www.researchgate.net\/profile\/Bharathi_Bethapudi2\/publication\/322324158_Polar_extract_of_Curcuma_longa_protects_cartilage_homeostasis_possible_mechanism_of_action\/links\/5aa9eddc0f7e9b88266f6d61\/Polar-extract-of-Curcuma-longa-protects-cartilage-homeostasis-possible-mechanism-of-action.pdf\"\u003eChandrasekaran Chinampudur Velusami, Edwin Jothie Richard \u0026amp;\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0024320519302383\"\u003e1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 0.054ng\/mL \/ Homo sapiens (Human)","offer_id":42481723015402,"sku":"ABCC24738","price":700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_11ab06a8-a00c-4250-8f5d-1b452e4fe605.png?v=1644865592","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-cytochrome-c-oxidase-subunit-ii-cox2-abcc24738","provider":"Dana Bioscience","version":"1.0","type":"link"}