{"product_id":"elisa-kit-for-complement-component-3-c3-abcc24684","title":"ELISA Kit for Complement Component 3 (C3)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e3.12-200ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eBos taurus; Bovine (Cattle)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 1.28ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complement Component 3 (C3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complement Component 3 (C3) and unlabeled Complement Component 3 (C3) (Standards or samples) with the pre-coated antibody specific to Complement Component 3 (C3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complement Component 3 (C3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complement Component 3 (C3) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eComplement Component 3\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eSignal transduction;Infection immunity;Immune molecule;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27032499\"\u003eOral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27173528\"\u003eComparative proteomics of milk fat globule membrane in goat colostrum and mature milk\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28888638\"\u003eThe response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain.\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 1.28ng\/mL \/ Bos taurus; Bovine (Cattle)","offer_id":42481711644906,"sku":"ABCC24684","price":756.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_dac88a44-5c62-49fe-893e-608c2d587629.png?v=1644865500","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-complement-component-3-c3-abcc24684","provider":"Dana Bioscience","version":"1.0","type":"link"}