{"product_id":"elisa-kit-for-alpha-1-antitrypsin-a1at-abcc22529","title":"ELISA Kit for Alpha-1-Antitrypsin (a1AT)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e781.25-200,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eCanis familiaris; Canine (Dog)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 353.94pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-1-Antitrypsin (a1AT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-1-Antitrypsin (a1AT) and unlabeled Alpha-1-Antitrypsin (a1AT) (Standards or samples) with the pre-coated antibody specific to Alpha-1-Antitrypsin (a1AT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-1-Antitrypsin (a1AT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-1-Antitrypsin (a1AT) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eSERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase\/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eAlpha-1-Antitrypsin\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eEnzyme \u0026amp; Kinase;Infection immunity;Hepatology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 353.94pg\/mL \/ Canis familiaris; Canine (Dog)","offer_id":42481441472746,"sku":"ABCC22529","price":760.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_daa129fc-88ff-4b96-a22f-66e4b8ee54a8.png?v=1644862092","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-alpha-1-antitrypsin-a1at-abcc22529","provider":"Dana Bioscience","version":"1.0","type":"link"}