{"product_id":"elisa-kit-for-adrenomedullin-adm-abcc25408","title":"ELISA Kit for Adrenomedullin (ADM)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e15.6-1,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eRattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 5.7pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenomedullin (ADM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenomedullin (ADM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenomedullin (ADM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenomedullin (ADM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eAM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eAdrenomedullin\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eEndocrinology;Hormone metabolism;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23660671\"\u003eShort-term hypoxia\/reoxygenation activates the angiogenic pathway in rat caudate putamen.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28501895\"\u003eAngiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0944711318302976\"\u003eComposition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failure\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 5.7pg\/mL \/ Rattus norvegicus (Rat)","offer_id":42481795596522,"sku":"ABCC25408","price":684.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_0fa30f4b-e340-4af3-b94b-c159ea284eb6.png?v=1644866566","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-adrenomedullin-adm-abcc25408","provider":"Dana Bioscience","version":"1.0","type":"link"}