{"product_id":"elisa-kit-for-5-hydroxyeicosatetraenoic-acid-5-hete-abcc24521","title":"ELISA Kit for 5-Hydroxyeicosatetraenoic Acid (5-HETE)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e9.88-800ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 3.87ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) and unlabeled 5-Hydroxyeicosatetraenoic Acid (5-HETE) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxyeicosatetraenoic Acid (5-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxyeicosatetraenoic Acid (5-HETE) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e \u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003e5-Hydroxyeicosatetraenoic Acid\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 3.87ng\/mL \/ Pan-species (General)","offer_id":42481685692650,"sku":"ABCC24521","price":816.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_564012ff-d49f-4a56-959e-bcf13f6bb18a.png?v=1644865235","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-5-hydroxyeicosatetraenoic-acid-5-hete-abcc24521","provider":"Dana Bioscience","version":"1.0","type":"link"}