{"product_id":"elisa-kit-for-12-hydroxyeicosatetraenoic-acid-12-hete-abcc24520","title":"ELISA Kit for 12-Hydroxyeicosatetraenoic Acid (12-HETE)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e12.35-1,000ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 5.12ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) and unlabeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) (Standards or samples) with the pre-coated antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e12HETE\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003e12-Hydroxyeicosatetraenoic Acid\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eSignal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.researchgate.net\/profile\/Pushkal_S_R3\/publication\/328335455_A_study_on_genotypic_frequency_of_12-lipoxygenase_polymorphisms_and_12-HETE_levels_in_type_II_diabetes\/links\/5bc71517458515f7d9bfe5e7\/A-study-on-genotypic-frequency-of-12-lipoxygenase-polymorphisms-and-12-HETE-levels-in-type-II-\"\u003eA study on genotypic frequency of 12-lipoxygenase polymorphisms and 12-HETE levels in type II diabetes\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 5.12ng\/mL \/ Pan-species (General)","offer_id":42481685659882,"sku":"ABCC24520","price":808.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_072cf81a-be84-4108-8ab5-1f5889596da1.png?v=1644865234","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-12-hydroxyeicosatetraenoic-acid-12-hete-abcc24520","provider":"Dana Bioscience","version":"1.0","type":"link"}