{"product_id":"elisa-kit-for-1-25-dihydroxyvitamin-d3-dhvd3-abcc25158","title":"ELISA Kit for 1,25-Dihydroxyvitamin D3 (DHVD3)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e24.69-2,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 9.92pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to DHVD3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled DHVD3 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of DHVD3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of DHVD3 in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eDH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003e1,25-Dihydroxyvitamin D3\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eMetabolic pathway;Bone metabolism;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3785422\/\"\u003eSelective Cytotoxic Action and DNA Damage by Calcitriol-Cu(II) Interaction: Putative Mechanism of Cancer Prevention\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3652781\/\"\u003eCalcium-deficiency assessment and biomarker identification by an integrated urinary metabonomics analysis\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24875663\"\u003eTreatment of established left ventricular hypertrophy with fibroblast growth factor receptor blockade in an animal model of CKD\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25188527\"\u003eVitamin D Prevents Podocyte Injury via Regulation of Macrophage M1\/M2 Phenotype in Diabetic Nephropathy Rats\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25547436\"\u003eCalcitriol?induced?redox?imbalance and DNA breakage in cells sharing a common metabolic feature of malignancies: Interaction with cellular copper (II) ions leads to the production of reactive oxygen species.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.researchgate.net\/profile\/Etimad_Huwait\/publication\/303311214_Impact_of_Curcumin_and_Flaxseed_Oil_on_Bone_Turnover_in_Glucocorticoid-Injected_Female_Rats\/links\/573c625b08ae298602e581c5.pdf\"\u003eImpact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats\u003c\/a\u003e;\u003ca href=\"\"\u003eProtective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats\u003c\/a\u003e;\u003ca href=\"http:\/\/link.springer.com\/article\/10.1007\/s10495-016-1261-2\"\u003eCalcitriol–copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular …\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28064234\"\u003eEffect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29093562\"\u003eGlucocorticoid exposure induces preeclampsia via dampening 1,25-dihydroxyvitamin D3\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ijcem.com\/files\/ijcem0064848.pdf\"\u003eCombined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2214623719300444\"\u003eDobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 9.92pg\/mL \/ Pan-species (General)","offer_id":42481770791146,"sku":"ABCC25158","price":796.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_53b1018c-49f5-4a0c-9ffd-3f20c3b207a0.png?v=1644866206","url":"https:\/\/danabiosci.com\/products\/elisa-kit-for-1-25-dihydroxyvitamin-d3-dhvd3-abcc25158","provider":"Dana Bioscience","version":"1.0","type":"link"}