{"product_id":"cusabio-csb-pa015988la01hu-50ug","title":"NPHS1 Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2022-04-01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-PA015988LA01HU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eNPHS1 Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003ePolyclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$299\/100ug, $166\/50ug\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, IHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:1000-1:3000, IHC:1:100-1:300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eAntigen affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003ePBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium Azide, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eA synthesized peptide derived from human Nephrin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniprot ID\u003c\/th\u003e\n\u003ctd\u003eO60500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eNPHS1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/1090438\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e50ug\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003ePositive WB detected in: JK whole cell lysate30µg), U251 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), 293T whole cell lysate(30µg)\u003cbr\u003e\u003cbr\u003eAll lanes: NPHS1 antibody at 1:1000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to rabbit IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 135,131 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 135 kDa\u003cbr\u003e\u003cbr\u003eExposure time：120s\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eIHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.\u003cbr\u003eSecondary antibody only control: uses 1% BSA instead of primary antibody\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eIHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.\u003cbr\u003eSecondary antibody only control: uses 1% BSA instead of primary antibody\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"50ug","offer_id":53121062699288,"sku":"CSB-PA015988LA01HU","price":166.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/CSB-PA015988LA01HU-WB_b437b24c-e3b0-405b-a631-a7b501820a5f.jpg?v=1793602529","url":"https:\/\/danabiosci.com\/products\/cusabio-csb-pa015988la01hu-50ug","provider":"Dana Bioscience","version":"1.0","type":"link"}