{"product_id":"cusabio-csb-pa010389la01hu-100ug","title":"HIST1H2AG Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2022-04-01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-PA010389LA01HU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eHIST1H2AG Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003ePolyclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$299\/100ug, $166\/50ug\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, IHC, IF, ChIP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eProtein G purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003e0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eRecombinant human Histone H2A type 1 protein (2-130aa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniprot ID\u003c\/th\u003e\n\u003ctd\u003eP0C0S8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eHIST1H2AG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/11106201\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100ug\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003eWestern blot\u003cbr\u003e\u003cbr\u003eAll lanes: HIST1H2AG antibody at 4µg\/ml + HL60 whole cell lysate\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to rabbit IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 15 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 15 kDa\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eImmunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010389LA01HU at dilution of 1:100\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eImmunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010389LA01HU at dilution of 1:100\u003c\/p\u003e\u003ch3\u003eImage 4\u003c\/h3\u003e\u003cp\u003eChromatin Immunoprecipitation Hela(1.2*10\u0026lt;sup\u0026gt;6\u0026lt;\/sup\u0026gt;)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010389HU) against the PGK1 promoter.\u003c\/p\u003e\u003ch3\u003eImage 5\u003c\/h3\u003e\u003cp\u003eImmunofluorescent analysis of Hela cells using CSB-PA010389LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"100ug","offer_id":53120889323800,"sku":"CSB-PA010389LA01HU","price":299.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/CSB-PA010389LA01HU-WB_09f6f93d-3c2c-4464-8be4-aab99dc95817.jpg?v=1793599362","url":"https:\/\/danabiosci.com\/products\/cusabio-csb-pa010389la01hu-100ug","provider":"Dana Bioscience","version":"1.0","type":"link"}