{"product_id":"clia-kit-for-neuropeptide-ff-npff-abcc22976","title":"CLIA Kit for Neuropeptide FF (NPFF)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e12.35-1,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eChemiluminescent immunoassay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 4.99pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Neuropeptide FF (NPFF). A competitive inhibition reaction is launched between biotin labeled Neuropeptide FF (NPFF) and unlabeled Neuropeptide FF (NPFF) (Standards or samples) with the pre-coated antibody specific to Neuropeptide FF (NPFF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide FF (NPFF) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Neuropeptide FF (NPFF) level in the sample or standard. \u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eFMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eNeuropeptide FF\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eNeuro science;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 4.99pg\/mL \/ Homo sapiens (Human)","offer_id":42481495638250,"sku":"ABCC22976","price":840.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_8aa2f1bc-1dfd-4d0a-924c-8d8bbe08e6dd.png?v=1644862731","url":"https:\/\/danabiosci.com\/products\/clia-kit-for-neuropeptide-ff-npff-abcc22976","provider":"Dana Bioscience","version":"1.0","type":"link"}