{"product_id":"clia-kit-for-5-hydroxytryptamine-5-ht-abcc22296","title":"CLIA Kit for 5-Hydroxytryptamine (5-HT)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e3.52-900ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eChemiluminescent immunoassay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003ePan-species (General)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 1.54ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eserum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe microplate provided in this kit has been pre-coated with a monoclonal antibody specific to 5-Hydroxytryptamine (5-HT). A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptamine (5-HT) and unlabeled 5-Hydroxytryptamine (5-HT) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptamine (5-HT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the 5-Hydroxytryptamine (5-HT) level in the sample or standard. \u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003e5HT; Serotonin; N-Methyl-Gamma\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003e5-Hydroxytryptamine\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eSignal transduction;Infection immunity;Neuro science;Gastroenterology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 1.54ng\/mL \/ Pan-species (General)","offer_id":42481408966890,"sku":"ABCC22296","price":955.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_3979d1c8-ed02-44b2-919c-1bf78d1a2937.png?v=1644861796","url":"https:\/\/danabiosci.com\/products\/clia-kit-for-5-hydroxytryptamine-5-ht-abcc22296","provider":"Dana Bioscience","version":"1.0","type":"link"}