{"product_id":"c13005-k01","title":"Double-Stranded RNA (dsRNA) ELISA Kit, J2-Based – Quantitative IVT RNA QC","description":"\u003cdiv style=\"max-width:900px;margin:0 auto;font-family:Arial,Helvetica,sans-serif;color:#1f2937;line-height:1.75;\"\u003e\n\n  \u003c!-- HERO --\u003e\n  \u003cdiv style=\"padding:32px;background:linear-gradient(135deg,#effaf7,#f5fbfa);border-radius:18px;margin-bottom:34px;\"\u003e\n\n    \u003cdiv style=\"font-size:13px;font-weight:700;color:#087c75;letter-spacing:.06em;\"\u003e\n      02 — dsRNA IMPURITY\n    \u003c\/div\u003e\n\n    \u003ch2 style=\"font-size:30px;line-height:1.3;margin:8px 0 12px;color:#111827;\"\u003e\n      Quantify Residual dsRNA in IVT RNA Samples\n    \u003c\/h2\u003e\n\n    \u003cp style=\"font-size:18px;margin:0;\"\u003e\n      A J2-based sandwich ELISA for quantitative detection of\n      \u003cstrong\u003edouble-stranded RNA (dsRNA)\u003c\/strong\u003e in IVT RNA research workflows.\n    \u003c\/p\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- QUICK SPECS --\u003e\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(170px,1fr));gap:14px;margin:30px 0;\"\u003e\n\n    \u003cdiv style=\"padding:20px;background:#f8fafc;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#64748b;font-weight:700;\"\u003eFORMAT\u003c\/div\u003e\n      \u003cstrong style=\"font-size:19px;\"\u003e96 Wells\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;background:#f8fafc;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#64748b;font-weight:700;\"\u003eASSAY\u003c\/div\u003e\n      \u003cstrong style=\"font-size:19px;\"\u003eSandwich ELISA\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;background:#f8fafc;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#64748b;font-weight:700;\"\u003eANTIBODY\u003c\/div\u003e\n      \u003cstrong style=\"font-size:19px;\"\u003eJ2-Based\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;background:#f8fafc;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#64748b;font-weight:700;\"\u003eSTORAGE\u003c\/div\u003e\n      \u003cstrong style=\"font-size:19px;\"\u003e2–8°C\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- WHY DSRNA --\u003e\n  \u003ch2 style=\"margin-top:48px;font-size:27px;color:#111827;\"\u003e\n    RNA Size Is Only Part of IVT RNA QC\n  \u003c\/h2\u003e\n\n  \u003cp\u003e\n    Agarose gel electrophoresis can help determine whether an RNA sample\n    appears near the expected transcript size, but it does not quantitatively\n    measure residual double-stranded RNA.\n  \u003c\/p\u003e\n\n  \u003cp\u003e\n    dsRNA can arise as a byproduct during in vitro transcription and therefore\n    represents a separate analytical question from RNA size and integrity.\n  \u003c\/p\u003e\n\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(250px,1fr));gap:16px;margin:28px 0;\"\u003e\n\n    \u003cdiv style=\"padding:24px;background:#f3f6ff;border-radius:15px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#155eef;font-weight:700;\"\u003eRNA LADDER\u003c\/div\u003e\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003eSize \u0026amp; Integrity\u003c\/h3\u003e\n      \u003cp style=\"margin:0;\"\u003e\n        Is the transcript approximately the expected size?\n      \u003c\/p\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:24px;background:#f1faf7;border:2px solid #bce0da;border-radius:15px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#087c75;font-weight:700;\"\u003edsRNA ELISA\u003c\/div\u003e\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003eImpurity Analysis\u003c\/h3\u003e\n      \u003cp style=\"margin:0;\"\u003e\n        How much residual dsRNA is present in the sample?\n      \u003c\/p\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- ASSAY PRINCIPLE --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    J2-Based Quantitative Sandwich ELISA\n  \u003c\/h2\u003e\n\n  \u003cp\u003e\n    The assay uses an anti-dsRNA monoclonal antibody to capture dsRNA,\n    followed by HRP-conjugated anti-dsRNA detection.\n  \u003c\/p\u003e\n\n  \u003cdiv style=\"margin:30px 0;text-align:center;\"\u003e\n\n    \u003cdiv style=\"padding:18px;background:#f4f8f7;border-radius:13px;\"\u003e\n      \u003cstrong\u003e1. dsRNA Sample\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:24px;padding:4px;\"\u003e↓\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:18px;background:#edf8f5;border-radius:13px;\"\u003e\n      \u003cstrong\u003e2. Capture by Anti-dsRNA Antibody\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:24px;padding:4px;\"\u003e↓\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:18px;background:#e9f6f2;border-radius:13px;\"\u003e\n      \u003cstrong\u003e3. HRP-Conjugated Detection Antibody\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:24px;padding:4px;\"\u003e↓\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:18px;background:#087c75;color:white;border-radius:13px;\"\u003e\n      \u003cstrong\u003e4. OD450 Measurement \u0026amp; Standard Curve\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- APPLICATIONS --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    Applications\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(220px,1fr));gap:14px;margin:25px 0;\"\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #dce7e3;border-radius:14px;\"\u003e\n      \u003cstrong\u003eIVT mRNA\u003c\/strong\u003e\u003cbr\u003e\n      Quantitative comparison of residual dsRNA in IVT samples.\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #bce0da;background:#f5fbfa;border-radius:14px;\"\u003e\n      \u003cstrong style=\"color:#087c75;\"\u003esaRNA Research\u003c\/strong\u003e\u003cbr\u003e\n      Complementary impurity analysis for long self-amplifying RNA workflows.\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #dce7e3;border-radius:14px;\"\u003e\n      \u003cstrong\u003ePurification Development\u003c\/strong\u003e\u003cbr\u003e\n      Compare dsRNA levels before and after purification steps.\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #dce7e3;border-radius:14px;\"\u003e\n      \u003cstrong\u003eProcess Optimization\u003c\/strong\u003e\u003cbr\u003e\n      Compare experimental IVT or purification conditions.\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- PRE POST --\u003e\n  \u003ch2 style=\"margin-top:50px;font-size:27px;color:#111827;\"\u003e\n    A Practical Use Case: Before vs After Purification\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"display:grid;grid-template-columns:1fr auto 1fr;gap:15px;align-items:center;margin:28px 0;\"\u003e\n\n    \u003cdiv style=\"padding:24px;background:#f8fafc;border-radius:15px;text-align:center;\"\u003e\n      \u003cstrong\u003eIVT Reaction\u003c\/strong\u003e\u003cbr\u003e\n      Pre-purification sample\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:26px;\"\u003e→\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:24px;background:#effaf7;border-radius:15px;text-align:center;\"\u003e\n      \u003cstrong\u003ePurified RNA\u003c\/strong\u003e\u003cbr\u003e\n      Post-purification sample\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n  \u003cp\u003e\n    Measuring both samples can help researchers compare relative dsRNA levels\n    and evaluate the effect of purification or process changes.\n  \u003c\/p\u003e\n\n\n  \u003c!-- PRODUCT SPECS --\u003e\n  \u003ch2 style=\"margin-top:50px;font-size:27px;color:#111827;\"\u003e\n    Product Specifications\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"overflow-x:auto;margin:25px 0;\"\u003e\n    \u003ctable style=\"width:100%;border-collapse:collapse;min-width:560px;\"\u003e\n\n      \u003ctbody\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;width:35%;\"\u003e\u003cstrong\u003eCatalog Number\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eC13005-K01\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eDouble-Stranded RNA (dsRNA) ELISA Kit, J2-Based\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003e96 wells\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eMethod\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eDouble-antibody sandwich ELISA\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eTarget\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eDouble-stranded RNA\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eReadout\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eAbsorbance at 450 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;\"\u003e2–8°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n      \u003c\/tbody\u003e\n\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n\n  \u003c!-- FAMILY --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    Complete Your IVT RNA QC Workflow\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(230px,1fr));gap:17px;margin:27px 0;\"\u003e\n\n    \u003cdiv style=\"padding:25px;border:1px solid #cbd8ff;background:#f5f7ff;border-radius:16px;\"\u003e\n      \u003cdiv style=\"font-size:12px;font-weight:700;color:#155eef;\"\u003e01 — SIZE \u0026amp; INTEGRITY\u003c\/div\u003e\n\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003e12K RNA Ladder\u003c\/h3\u003e\n\n      \u003cp\u003e\n        High-range RNA size reference from 200 nt to 12 kb.\n      \u003c\/p\u003e\n\n      \u003ca href=\"\/products\/cr00005-50ul\" style=\"color:#155eef;font-weight:700;text-decoration:none;\"\u003e\n        View CR00005 →\n      \u003c\/a\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:25px;border:2px solid #98d4c7;background:#f1faf7;border-radius:16px;\"\u003e\n      \u003cdiv style=\"font-size:12px;font-weight:700;color:#087c75;\"\u003e02 — dsRNA IMPURITY\u003c\/div\u003e\n\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003edsRNA ELISA\u003c\/h3\u003e\n\n      \u003cp\u003e\n        Quantitative J2-based analysis of residual dsRNA.\n      \u003c\/p\u003e\n\n      \u003cstrong style=\"color:#087c75;\"\u003eYou are here\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:25px;border:1px solid #ddd1ee;background:#faf8fd;border-radius:16px;\"\u003e\n      \u003cdiv style=\"font-size:12px;font-weight:700;color:#6941a5;\"\u003e03 — PROCESS RESIDUAL\u003c\/div\u003e\n\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003eT7 RNA Polymerase ELISA\u003c\/h3\u003e\n\n      \u003cp\u003e\n        Quantitative analysis of residual T7 RNA polymerase.\n      \u003c\/p\u003e\n\n      \u003ca href=\"\/search?q=C13004-K01\" style=\"color:#6941a5;font-weight:700;text-decoration:none;\"\u003e\n        View C13004 →\n      \u003c\/a\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- EDUCATION --\u003e\n  \u003cdiv style=\"margin-top:48px;padding:29px;background:#f4f7fb;border-radius:18px;\"\u003e\n\n    \u003cdiv style=\"font-size:13px;color:#087c75;font-weight:700;\"\u003e\n      RNA QC RESOURCES\n    \u003c\/div\u003e\n\n    \u003ch3 style=\"font-size:22px;margin:8px 0 14px;\"\u003e\n      Learn More About dsRNA in IVT RNA\n    \u003c\/h3\u003e\n\n    \u003cp\u003e\n      Understand how dsRNA analysis fits alongside RNA size\n      and process-related QC.\n    \u003c\/p\u003e\n\n    \u003cp\u003e\n      \u003ca href=\"\/blogs\/resources\/dsrna-impurities-ivt-mrna-sarna\" style=\"color:#087c75;font-weight:700;text-decoration:none;\"\u003e\n        → Why dsRNA Impurities Matter in IVT mRNA \u0026amp; saRNA\n      \u003c\/a\u003e\n    \u003c\/p\u003e\n\n    \u003cp\u003e\n      \u003ca href=\"\/blogs\/resources\/ivt-mrna-qc-workflow\" style=\"color:#087c75;font-weight:700;text-decoration:none;\"\u003e\n        → A Practical IVT mRNA QC Workflow\n      \u003c\/a\u003e\n    \u003c\/p\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- FINAL CTA --\u003e\n  \u003cdiv style=\"margin-top:48px;padding:36px;background:linear-gradient(135deg,#075e59,#087c75);color:white;border-radius:20px;\"\u003e\n\n    \u003cdiv style=\"font-size:13px;font-weight:700;color:#c9ebe4;\"\u003e\n      DANA BIOSCIENCE RNA QC SOLUTIONS\n    \u003c\/div\u003e\n\n    \u003ch2 style=\"font-size:28px;color:white;margin:9px 0 12px;\"\u003e\n      Need Quantitative dsRNA Analysis?\n    \u003c\/h2\u003e\n\n    \u003cp style=\"color:white;\"\u003e\n      Build a complementary IVT RNA QC workflow combining RNA size,\n      dsRNA impurity and residual process-enzyme analysis.\n    \u003c\/p\u003e\n\n    \u003ca href=\"\/pages\/rna-qc-solutions\" style=\"display:inline-block;margin-top:10px;padding:13px 22px;background:white;color:#087c75;text-decoration:none;font-weight:700;border-radius:30px;\"\u003e\n      Explore RNA QC Solutions\n    \u003c\/a\u003e\n\n  \u003c\/div\u003e\n\n  \u003cp style=\"margin-top:28px;font-size:13px;color:#738096;\"\u003e\n    For Research Use Only. Not for diagnostic or therapeutic use.\n    Follow the manufacturer's product manual for assay conditions,\n    sample preparation and interpretation.\n  \u003c\/p\u003e\n\n\u003c\/div\u003e","brand":"Croyezbio","offers":[{"title":"96 wells","offer_id":51746208809240,"sku":"C13005-K01","price":1400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Croyezbio.png?v=1715231500","url":"https:\/\/danabiosci.com\/products\/c13005-k01","provider":"Dana Bioscience","version":"1.0","type":"link"}