{"product_id":"c13004-k01","title":"T7 RNA Polymerase ELISA Kit for IVT mRNA QC","description":"\u003cdiv style=\"max-width:900px;margin:0 auto;font-family:Arial,Helvetica,sans-serif;color:#1f2937;line-height:1.75;\"\u003e\n\n  \u003c!-- HERO --\u003e\n  \u003cdiv style=\"padding:32px;background:linear-gradient(135deg,#f7f3fb,#faf8fd);border-radius:18px;margin-bottom:34px;\"\u003e\n\n    \u003cdiv style=\"font-size:13px;font-weight:700;color:#6941a5;letter-spacing:.06em;\"\u003e\n      03 — PROCESS RESIDUAL\n    \u003c\/div\u003e\n\n    \u003ch2 style=\"font-size:30px;line-height:1.3;margin:8px 0 12px;color:#111827;\"\u003e\n      Quantify Residual T7 RNA Polymerase After IVT\n    \u003c\/h2\u003e\n\n    \u003cp style=\"font-size:18px;margin:0;\"\u003e\n      A sensitive sandwich ELISA for quantitative detection of\n      \u003cstrong\u003eT7 RNA Polymerase\u003c\/strong\u003e in research-use IVT RNA workflows.\n    \u003c\/p\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- PERFORMANCE NUMBERS --\u003e\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(170px,1fr));gap:14px;margin:30px 0;\"\u003e\n\n    \u003cdiv style=\"padding:22px;background:#faf8fd;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#6941a5;font-weight:700;\"\u003eLOD\u003c\/div\u003e\n      \u003cstrong style=\"font-size:23px;\"\u003e0.037 ng\/mL\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:22px;background:#faf8fd;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#6941a5;font-weight:700;\"\u003eLOQ\u003c\/div\u003e\n      \u003cstrong style=\"font-size:23px;\"\u003e0.124 ng\/mL\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:22px;background:#faf8fd;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#6941a5;font-weight:700;\"\u003eDETECTION RANGE\u003c\/div\u003e\n      \u003cstrong style=\"font-size:20px;\"\u003e0.15625–10 ng\/mL\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:22px;background:#faf8fd;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cdiv style=\"font-size:12px;color:#6941a5;font-weight:700;\"\u003eFORMAT\u003c\/div\u003e\n      \u003cstrong style=\"font-size:23px;\"\u003e96 Wells\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- WHY --\u003e\n  \u003ch2 style=\"margin-top:50px;font-size:27px;color:#111827;\"\u003e\n    Why Measure Residual T7 RNA Polymerase?\n  \u003c\/h2\u003e\n\n  \u003cp\u003e\n    T7 RNA polymerase is commonly used to generate RNA during\n    in vitro transcription.\n  \u003c\/p\u003e\n\n  \u003cp\u003e\n    After transcription and purification, researchers may want to evaluate\n    how much process enzyme remains in the sample as part of a broader\n    process-development or RNA QC workflow.\n  \u003c\/p\u003e\n\n  \u003cdiv style=\"padding:26px;background:#f7f3fb;border-left:5px solid #6941a5;border-radius:4px 15px 15px 4px;margin:28px 0;\"\u003e\n    \u003cstrong style=\"font-size:18px;\"\u003e\n      RNA size, dsRNA impurity and residual T7 polymerase answer three different QC questions.\n    \u003c\/strong\u003e\n  \u003c\/div\u003e\n\n\n  \u003c!-- PRINCIPLE --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    Sandwich ELISA for T7 RNA Polymerase\n  \u003c\/h2\u003e\n\n  \u003cp\u003e\n    The assay uses a monoclonal antibody to capture T7 RNA Polymerase,\n    followed by an HRP-conjugated detection antibody and colorimetric\n    measurement at 450 nm.\n  \u003c\/p\u003e\n\n  \u003cdiv style=\"margin:30px 0;text-align:center;\"\u003e\n\n    \u003cdiv style=\"padding:18px;background:#f8f6fb;border-radius:13px;\"\u003e\n      \u003cstrong\u003e1. Sample Containing T7 RNA Polymerase\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:24px;padding:4px;\"\u003e↓\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:18px;background:#f5f1fa;border-radius:13px;\"\u003e\n      \u003cstrong\u003e2. Capture Antibody Binding\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:24px;padding:4px;\"\u003e↓\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:18px;background:#f1ebf8;border-radius:13px;\"\u003e\n      \u003cstrong\u003e3. HRP-Conjugated Detection Antibody\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"font-size:24px;padding:4px;\"\u003e↓\u003c\/div\u003e\n\n    \u003cdiv style=\"padding:18px;background:#6941a5;color:white;border-radius:13px;\"\u003e\n      \u003cstrong\u003e4. OD450 Measurement \u0026amp; Quantification\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- USE CASES --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    Applications\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(220px,1fr));gap:14px;margin:25px 0;\"\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cstrong\u003eIVT Process Development\u003c\/strong\u003e\u003cbr\u003e\n      Evaluate residual T7 polymerase following transcription.\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #d5c3e8;background:#faf8fd;border-radius:14px;\"\u003e\n      \u003cstrong style=\"color:#6941a5;\"\u003ePurification Evaluation\u003c\/strong\u003e\u003cbr\u003e\n      Compare T7 polymerase levels before and after purification.\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cstrong\u003emRNA Research\u003c\/strong\u003e\u003cbr\u003e\n      Add residual enzyme analysis to an IVT mRNA QC workflow.\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:20px;border:1px solid #e2d8ef;border-radius:14px;\"\u003e\n      \u003cstrong\u003esaRNA Research\u003c\/strong\u003e\u003cbr\u003e\n      Complement size and impurity analysis for long RNA workflows.\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- RANGE --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    Assay Performance\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"padding:30px;background:#111827;color:white;border-radius:18px;margin:26px 0;\"\u003e\n\n    \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(180px,1fr));gap:22px;\"\u003e\n\n      \u003cdiv\u003e\n        \u003cdiv style=\"font-size:12px;color:#c9b7e0;font-weight:700;\"\u003eLIMIT OF DETECTION\u003c\/div\u003e\n        \u003cdiv style=\"font-size:25px;font-weight:700;margin-top:4px;\"\u003e0.037 ng\/mL\u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003cdiv\u003e\n        \u003cdiv style=\"font-size:12px;color:#c9b7e0;font-weight:700;\"\u003eLIMIT OF QUANTIFICATION\u003c\/div\u003e\n        \u003cdiv style=\"font-size:25px;font-weight:700;margin-top:4px;\"\u003e0.124 ng\/mL\u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003cdiv\u003e\n        \u003cdiv style=\"font-size:12px;color:#c9b7e0;font-weight:700;\"\u003eDETECTION RANGE\u003c\/div\u003e\n        \u003cdiv style=\"font-size:23px;font-weight:700;margin-top:4px;\"\u003e0.15625–10 ng\/mL\u003c\/div\u003e\n      \u003c\/div\u003e\n\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- PRODUCT SPECS --\u003e\n  \u003ch2 style=\"margin-top:50px;font-size:27px;color:#111827;\"\u003e\n    Product Specifications\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"overflow-x:auto;margin:25px 0;\"\u003e\n    \u003ctable style=\"width:100%;border-collapse:collapse;min-width:560px;\"\u003e\n\n      \u003ctbody\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;width:35%;\"\u003e\u003cstrong\u003eCatalog Number\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eC13004-K01\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eT7 RNA Polymerase ELISA Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003ePre-coated 96-well microplate (12 × 8-well strips)\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eAssay\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eSandwich ELISA\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eTarget\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003eT7 RNA Polymerase\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eLOD\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003e0.037 ng\/mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eLOQ\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003e0.124 ng\/mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eDetection Range\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003e0.15625–10 ng\/mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;border-bottom:1px solid #e2e8f0;\"\u003e\u003cstrong\u003eReadout\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;border-bottom:1px solid #e2e8f0;\"\u003e450 nm\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n        \u003ctr\u003e\n          \u003ctd style=\"padding:14px;background:#f8fafc;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n          \u003ctd style=\"padding:14px;\"\u003e2–8°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n\n      \u003c\/tbody\u003e\n\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n\n\n  \u003c!-- FAMILY --\u003e\n  \u003ch2 style=\"margin-top:52px;font-size:27px;color:#111827;\"\u003e\n    Complete Your IVT RNA QC Workflow\n  \u003c\/h2\u003e\n\n  \u003cdiv style=\"display:grid;grid-template-columns:repeat(auto-fit,minmax(230px,1fr));gap:17px;margin:27px 0;\"\u003e\n\n    \u003cdiv style=\"padding:25px;border:1px solid #cbd8ff;background:#f5f7ff;border-radius:16px;\"\u003e\n      \u003cdiv style=\"font-size:12px;font-weight:700;color:#155eef;\"\u003e01 — SIZE \u0026amp; INTEGRITY\u003c\/div\u003e\n\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003e12K RNA Ladder\u003c\/h3\u003e\n\n      \u003cp\u003e\n        Evaluate RNA size with high-range reference bands.\n      \u003c\/p\u003e\n\n      \u003ca href=\"\/products\/cr00005-50ul\" style=\"color:#155eef;font-weight:700;text-decoration:none;\"\u003e\n        View CR00005 →\n      \u003c\/a\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:25px;border:1px solid #bce0da;background:#f4fbf9;border-radius:16px;\"\u003e\n      \u003cdiv style=\"font-size:12px;font-weight:700;color:#087c75;\"\u003e02 — dsRNA IMPURITY\u003c\/div\u003e\n\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003edsRNA ELISA\u003c\/h3\u003e\n\n      \u003cp\u003e\n        Quantitatively evaluate residual double-stranded RNA.\n      \u003c\/p\u003e\n\n      \u003ca href=\"\/search?q=C13005-K01\" style=\"color:#087c75;font-weight:700;text-decoration:none;\"\u003e\n        View C13005 →\n      \u003c\/a\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv style=\"padding:25px;border:2px solid #cfbae5;background:#f8f4fc;border-radius:16px;\"\u003e\n      \u003cdiv style=\"font-size:12px;font-weight:700;color:#6941a5;\"\u003e03 — PROCESS RESIDUAL\u003c\/div\u003e\n\n      \u003ch3 style=\"font-size:20px;margin:7px 0;\"\u003eT7 RNA Polymerase ELISA\u003c\/h3\u003e\n\n      \u003cp\u003e\n        Quantitative residual enzyme analysis following IVT.\n      \u003c\/p\u003e\n\n      \u003cstrong style=\"color:#6941a5;\"\u003eYou are here\u003c\/strong\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- CONTENT LINK --\u003e\n  \u003cdiv style=\"margin-top:48px;padding:29px;background:#f4f7fb;border-radius:18px;\"\u003e\n\n    \u003cdiv style=\"font-size:13px;color:#6941a5;font-weight:700;\"\u003e\n      RNA QC RESOURCES\n    \u003c\/div\u003e\n\n    \u003ch3 style=\"font-size:22px;margin:8px 0 14px;\"\u003e\n      Build a Practical IVT RNA QC Workflow\n    \u003c\/h3\u003e\n\n    \u003cp\u003e\n      See how RNA size, dsRNA impurity and residual T7 RNA polymerase\n      can be evaluated as complementary QC questions.\n    \u003c\/p\u003e\n\n    \u003ca href=\"\/blogs\/resources\/ivt-mrna-qc-workflow\" style=\"color:#6941a5;font-weight:700;text-decoration:none;\"\u003e\n      → Read the IVT mRNA QC Workflow Guide\n    \u003c\/a\u003e\n\n  \u003c\/div\u003e\n\n\n  \u003c!-- CTA --\u003e\n  \u003cdiv style=\"margin-top:48px;padding:36px;background:linear-gradient(135deg,#4f2c78,#6941a5);color:white;border-radius:20px;\"\u003e\n\n    \u003cdiv style=\"font-size:13px;font-weight:700;color:#dfd1ef;\"\u003e\n      DANA BIOSCIENCE RNA QC SOLUTIONS\n    \u003c\/div\u003e\n\n    \u003ch2 style=\"font-size:28px;color:white;margin:9px 0 12px;\"\u003e\n      Add Residual Enzyme Analysis to Your IVT Workflow\n    \u003c\/h2\u003e\n\n    \u003cp style=\"color:white;\"\u003e\n      Combine T7 RNA polymerase analysis with RNA size\n      and dsRNA impurity assessment for a broader view of your IVT RNA process.\n    \u003c\/p\u003e\n\n    \u003ca href=\"\/pages\/rna-qc-solutions\" style=\"display:inline-block;margin-top:10px;padding:13px 22px;background:white;color:#6941a5;text-decoration:none;font-weight:700;border-radius:30px;\"\u003e\n      Explore RNA QC Solutions\n    \u003c\/a\u003e\n\n  \u003c\/div\u003e\n\n  \u003cp style=\"margin-top:28px;font-size:13px;color:#738096;\"\u003e\n    For Research Use Only. Not for diagnostic or therapeutic use.\n    Follow the manufacturer's product manual for assay conditions,\n    sample preparation and interpretation.\n  \u003c\/p\u003e\n\n\u003c\/div\u003e","brand":"Croyezbio","offers":[{"title":"96 wells","offer_id":48995685302552,"sku":"C13004-K01","price":1584.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Croyezbio.png?v=1715231500","url":"https:\/\/danabiosci.com\/products\/c13004-k01","provider":"Dana Bioscience","version":"1.0","type":"link"}