{"product_id":"biotinylated-ssrna-ladder","title":"Biotinylated ssRNA Ladder","description":"\u003cdiv class=\"pd-dt-top\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"col-sm-7 fck\"\u003eThe Biotinylated ssRNA Ladder, comprising eight biotin-labeled single-stranded RNAs (0.2k, 0.5k, 1k, 2k, 3k, 4k, 6k, and 9k bases), is synthetically generated through in vitro transcription from a blend of eight linear DNA templates, with biotin-labeled nucleotides incorporated during transcription. This ladder serves as an effective tool for determining the size of single-stranded RNAs in Northern blot analysis and in non-denaturing agarose gel electrophoresis. After transfer, the biotin-labeled bands are detected directly with streptavidin-HRP and a chemiluminescent substrate (ECL), without probe hybridization. Before transfer, the ladder can also be visualized by UV light post-ethidium bromide or nucleic acid safety dye staining.\u003c\/div\u003e\n\u003cdiv class=\"col-sm-7 fck\"\u003e\u003c\/div\u003e\n\u003cdiv class=\"col-sm-7 fck\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e\n\u003cstrong\u003eQuality Control Testing\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eThe banding pattern of the Biotin ssRNA Ladder on 1% TAE agarose gels shows clear identifiable bands at each fragment, when stained with nucleic acid safety dye under UV light. After SSC capillary transfer, all eight bands are detected by streptavidin-HRP and ECL.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003e1 mM sodium citrate buffer (pH 6.4)\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage \u0026amp; Stability\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eStorage at -80°C and avoid repeated freeze\/thaw cycles.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eHandling Instruction\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eFor optimal storage, aliquot the reagent into smaller quantities and store at recommended temperature.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003ePlease promptly retrieve the required portion and return it to the appropriate storage temperature.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRecommended to Use\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eLadder preparation for Electrophoresis\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003e1. Mix 1 volume of RNA ladder with 3 volumes of 2X RNA Loading Dye.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e2. Incubate at 65°C for 5 minutes.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e3. Immediately place it on ice for 1-2 minutes\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e4. Load 4 μL of the prepared mixture per lane (1 μg of ladder at 1 μg\/μL;Figure 1).\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eNorthern Blot Detection\u003cbr\u003eConditions used in Figure 1\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003e1. Separate the prepared ladder on a 1% agarose\/TAE gel (1 μg\/lane).\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e2. Transfer to a positively charged nylon membrane by SSC capillary transfer (6 h), then bake at 60°C for 30 minutes.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e3. Block with 5% BSA in TBST (pH 7.5) for 1 h, incubate with streptavidin-HRP (1:10,000 in blocking buffer) for 1 h, and wash 4 times with TBST.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e4. Develop with ECL substrate and image (10 s exposure in Figure 1).\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eNorthern blot; Electrophoresis\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eNote\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003e1. To avoid ribonuclease contamination, using RNase-free water is necessary for sample dilution.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e2. We do not recommend use of these markers as a quantitative standard. Relative band intensities differ among fragments, and between the stained gel and the chemiluminescent blot.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eShipping Conditions\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eDry ice\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Croyezbio","offers":[{"title":"Default Title","offer_id":53167100821784,"sku":"CR00049-25UL","price":400.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/094155520.png?v=1793960227","url":"https:\/\/danabiosci.com\/products\/biotinylated-ssrna-ladder","provider":"Dana Bioscience","version":"1.0","type":"link"}