{"product_id":"bek1345","title":"Human B7-1\/CD80 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of B7-1 \/ CD80 in human serum, plasma, body fluids, tissue lysatesor cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e The Cluster of differentiation 80 (also CD80 and B7-1) is a B7, type I membrane proteinin the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is closely related to CD86, another B7 protein (B7-2), and often works in tandem. Both CD80 and CD86 interact with costimulatory receptors CD28, CTLA-4 (CD152) and the p75 neurotrophin receptor.CD80 is a member of the B7 family, which consists of molecules present at APCs and their receptors present on the T-cells.[7] CD80 is present specifically on DC, activated B-cells, and macrophages, but also T-cells. CD80 is also a transmembrane glycoprotein and a member of the Ig superfamily.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-B7-1 \/ CD80 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-1 \/ CD80 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theB7-1 \/ CD80amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofB7-1 \/ CD80can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 62.5 pg\/ml - 4000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt;12 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409053782296,"sku":"BEK1345","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_b510e3f7-df33-4d56-a8eb-91a65002f40c.jpg?v=1750988959","url":"https:\/\/danabiosci.com\/products\/bek1345","provider":"Dana Bioscience","version":"1.0","type":"link"}